In this study, to improve the performance of a solid oxide fuel cell based on a porous metal support, a fuel cell using a multi-layered anode functional layer was fabricated and electrochemical performance analysis was performed. Surface and cross-sectional microstructures according to particle size control were confirmed through FE-SEM. The pore size of the multi-layer anode functional layer was gradually reduced compared to that of a single-structure anode functional layer. As a result, it was confirmed that the surface roughness was lower than that of the single structure. This led to a reduction in polarization resistance through smooth transmission of gas generated from the electrode. As a result, it was confirmed that electrochemical performance was improved by more than 1.25 times in fuel cells using a multi-layered anode functional layer compared to that with a single structure.