Maintaining balance is a factor critical and integral to our effective physical function as it relates to the normal activities of daily living. Of the “hip strategy”, “stepping strategy” and “ankle strategy”, it is known and accepted that the “ankle strategy” is the first activated parameter to assist in the maintenance of balance in motor output. However, few studies actually evaluated or assessed the precise attributes of “ankle strategy” in relation to any therapeutic effort tocorrect and/or rehabilitate from physical imbalance caused by dynamic tilting perturbation. The aim of the study was to identify precise characteristics of the “ankle strategy” as they respond to dynamic tilting perturbations. Seven healthy male (aged 25.5 ± 1.7 years, average height of 173.9 ± 6.4 cm, average body mass of 71.3 ± 6.5 kg) were recruited to participate. The ankle joint motions were subjected to eight dynamic tilting perturbations generated by the customized tilting perturbation simulator, and the responses were measured by 3D motion capture system. Concurrently, foot pressure distribution and the corresponding centers of pressure (COP) trajectory were measured by a pressure measuring system, and the four main muscles’ activations related to the ankle joint motions were measured by wireless electromyogram system.