In this study, a highly sensitive analysis device for hydrogen sulfide that could be used quickly and easily on site was developed using a colorimetric paper sensor. To optimize analysis conditions, tests were performed for each function. Performances of the method using laboratory equipment and tools and the method using the developed device for hydrogen sulfide analysis were compared. The trend line of changes in parameter b of the image acquired by the on-site analytical device for hydrogen sulfide was calculated as y = 0.517x - 0.141 with a coefficient of determination (R2) of 0.9874. It was comparable to the method performed at the laboratory level, showing an excellent linearity. Using the calculated trend line as a calibration curve, the detection limit and quantification limit were found to be 2.386 μM and 7.952 μM, respectively. A reproducibility test showed a relative standard deviation of 5.7%, indicating a low dispersion of results.