Microfluidics allows for precise manipulation of small volumes of analytical solutions in diverse applications, including disease diagnostics, drug efficacy testing, chemical analysis, and water quality monitoring. Among these diverse applications, one of the most critical aspects is the precise and programmable control of flow within microfluidic control devices. However, microfluidic experiments that employ pressure control via a gas tank may encounter restricted mobility. To address these challenges, we developed an air pump feedback control system utilizing artificial intelligence image analysis and devised a method to enhance portability. In this paper, we utilized a commercially available portable pump to achieve the desired pressure and subsequently cease operation. In addressing the challenge of sustaining prolonged pressure, we implemented a strategy wherein the dimensions of the pressure vessel were modified, accompanied by iterative pump activations, thereby ensuring the sustained maintenance of pressure over time. The evaluation of the flow controller developed in this study involves conducting a comparative flow analysis with established pneumatic flow controllers. Furthermore, we employed artificial intelligence image analysis methods to automate the operation of iterative pumps. In conclusion, we anticipate that the developed portable microfluidic control device will lead to innovative advancements in modern technology and healthcare through its potential applications.