Scaffolds for bone tissue engineering (BTE) should accomplish appropriate mechanical, cell interaction, and new bone ingrowth properties. Among calcium phosphate (CaP) based bio-ceramics used for preparing scaffolds, biphasic calcium phosphate (BCP) is attracting great interest for fabricating BTE scaffolds owing to its excellent biocompatibility and osteoconductivity. Fused deposition modeling (FDM) is an additive manufacturing technology commonly used for modeling, prototyping, and production applications. It is one of techniques used for 3D printing. The main purpose of this study was to develop new fabrication process of BCP scaffolds based on extrusion moulding using a 3D printer. Through the 3D printer, we showed new fabrication process for making scaffold mould and extrusion device parts that could be combined with tension-compression test machine. Line width, pore size, and porosity of these fabricated BCP scaffolds were measured and calculated. Mechanical properties and cell proliferation results of these BCP scaffolds were then evaluated.