A femtosecond laser is used in various fields such as microscale machining, OLED repair, micro 3D structure fabrication, and eye surgery. Particularly, because of non-thermal property, ablation and ablation threshold are the most representative characteristics of femtosecond laser. The ablation system is accompanied by many optics, stage, or gantry. In the case of the gantry, an ordinary optic system delivers a beam where mirrors and lens are required. If the gantry moves to the sample, external stimulation such as vibration will occur. Vibration has an influence on optics such as transforming beam path and becomes an error that decreases accuracy, precision, and spatial resolution. Generally, Fiber Optic Beam Delivery System (FOBDS) is used to solve this issue. But in the case of the femtosecond laser, FOBDS is incompatible. Recently, another FOBDS model that is compatible with the femtosecond laser has been developed. In this paper, the ablation threshold was obtained by FOBDS and femtosecond laser. The results present a possibility of ablation without external stimulation.