Farming is a typical task that includes repetitive tasks, incomplete working positions, and weight work, along with exposure to a number of musculoskeletal diseases and harmful factors. Therefore, in this study, work clothes were developed for older agricultural workers exposed to musculoskeletal diseases and alienated from the medical system. Work clothes can help in repetitive cropping and support the load on the work, preventing and mitigating farmers" diseases. To verify the effectiveness of the developed clothing, six men in their 20s were evaluated for muscle usage before and after wearing the clothing in two ways: stoop lifting and squat lifting. The results of this study showed that the mass of most muscles, except the gastrocnemius muscle, was reduced during lifting operations by stoop lifting after wearing work clothes. The reason why the gastrocnemius was more activated was that the ankle joint was greatly activated by dorsal flexion. For squatting and lifting, most of the muscle usage was reduced. In future research, we want to analyze the muscle characteristics of actual agricultural workers on wearing work clothes.