Microfluidic chips have become a critical component in advanced applications such as biochemical analysis, medical diagnostics, drug development, and environmental monitoring because of their ability to precisely control fluid flow at the microscale. The functionality of these chips is highly dependent on the precision and dimensional stability of microchannel structures formed on them. While injection molding is an efficient method for a mass production of microfluidic chips, it is required to minimize undesirable deformation due to thermal and mechanical stresses, which can degrade the overall performance. This study investigated global (Macro-scale) and local (Micro-scale) deformation behaviors of injection-molded microfluidic chips. Effects of processing parameters, including mold temperature, melt temperature, filling time, and packing pressure, were investigated. The Taguchi-based design of experiments approach was employed to systematically analyze these effects and to determine optimal conditions to minimize deformation.