Microfluidic chips have become a critical component in advanced applications such as biochemical analysis, medical diagnostics, drug development, and environmental monitoring because of their ability to precisely control fluid flow at the microscale. The functionality of these chips is highly dependent on the precision and dimensional stability of microchannel structures formed on them. While injection molding is an efficient method for a mass production of microfluidic chips, it is required to minimize undesirable deformation due to thermal and mechanical stresses, which can degrade the overall performance. This study investigated global (Macro-scale) and local (Micro-scale) deformation behaviors of injection-molded microfluidic chips. Effects of processing parameters, including mold temperature, melt temperature, filling time, and packing pressure, were investigated. The Taguchi-based design of experiments approach was employed to systematically analyze these effects and to determine optimal conditions to minimize deformation.

Microfluidics allows for precise manipulation of small volumes of analytical solutions in diverse applications, including disease diagnostics, drug efficacy testing, chemical analysis, and water quality monitoring. Among these diverse applications, one of the most critical aspects is the precise and programmable control of flow within microfluidic control devices. However, microfluidic experiments that employ pressure control via a gas tank may encounter restricted mobility. To address these challenges, we developed an air pump feedback control system utilizing artificial intelligence image analysis and devised a method to enhance portability. In this paper, we utilized a commercially available portable pump to achieve the desired pressure and subsequently cease operation. In addressing the challenge of sustaining prolonged pressure, we implemented a strategy wherein the dimensions of the pressure vessel were modified, accompanied by iterative pump activations, thereby ensuring the sustained maintenance of pressure over time. The evaluation of the flow controller developed in this study involves conducting a comparative flow analysis with established pneumatic flow controllers. Furthermore, we employed artificial intelligence image analysis methods to automate the operation of iterative pumps. In conclusion, we anticipate that the developed portable microfluidic control device will lead to innovative advancements in modern technology and healthcare through its potential applications.

Advances in cell culture technology have improved the understanding of the physiological principles of cells. Recently, the development of microfluidic chips has made it possible to observe single cells in a massively parallelized and accurate manner. However, in order to maximize the availability of the microfluidic cell chip, it is essential to use an incubator that can isolate the cell culture chip from the outside while minimizing contamination and maintaining the temperature and humidity required for cell culture for a long time period. Here, we developed a thermo-hygrostat incubator consisting of an Arduino-based feedback control module for controlling a temperature and humidity complex sensor, a humidifier, and a heater. The temperature and humidity of the incubator could be actively changed according to the needs and application by simple editing control variables of Arduino coding. To demonstrate the efficiency of the device, we conducted an experiment comparing the growth of bacterial cells and obtained optimal conditions necessary for culture. In conclusion, it is expected that the newly developed thermo-hygrostat incubator can be used for a variety of purposes that require active control of temperature and humidity, as well as for long-term cultivation of bacterial cells inside a microfluidic chip.

In this paper, theoretical and experimental studies were conducted on the cooling performance of a microchannel heat dissipation device with a manifold layer added. By adding 500 μm wide microchannels and manifold flow fields, the rheological properties of the cooling fluid were enhanced to improve the heat transfer performance. The size of the microchannel used for cooling was 40 × 40 × 5 mm, and was evaluated under a heat flux of 12.5-43.75 W/㎠ and a flow rate of 0.3-1.1 L /min conditions. As a result of the experiment, in the case of a microchannel heat sink of 500 μm compared to the existing heat sink, cooling was successfully performed under a heat flux condition of about four times

In this paper, a microfluidic co-culture system comprising an embedded polydimethylsiloxane (PDMS) microstencil was fabricated. The fabricated co-culture system has two micro-channels separated with a PDMS microstencil membrane. Master molds for microchannels and stencil membranes were fabricated by photolithography, then used for casting of PDMS devices. The stencil membrane was 10 thick, with holes 10-μm large in diameter. The fabricated system co-cultured two types of cells (HepG2, NIH-3T3 Cells) successfully for seven days. The viability and stability of the cells were verified through LIVE/DEAD® staining and analysis. Additionally, albumin secretion of HepG2 cells was measured for seven days, using an HSA ELISA kit. The measured data were analyzed, to compare the activity of HepG2 cells. Results confirmed that cells can be co-cultured in the fabricated microfluidic system.

In this study, we present the multilayered symmetrical droplet splitting microfluidic system for preparation of microspheres. The microfluidic device was fabricated by conventional photolithography and PDMS casting. Multiple layers of microfluidic channels for symmetrical droplet splitting were stacked and integrated into a device. Each layer was designed to obtain 16 microdroplets from one droplet by droplet splitting. The droplet size was controlled with flow rate of dispersed phase (DI-water) and continuous phase (Mineral Oil with 3 wt.% SPAN80) by using a syringe pump. The droplet splitting behavior and production rate were analyzed by high-speed camera and inverted microscope in one layer of the microfluidic device. Additionally, the droplet size and size distribution were observed in each layer of the microfluidic device. The droplet size could be controlled by flow control of two phase flows with high uniformity of droplet size less than 5% coefficient of variation.

We present a multi-sample array device based on a pneumatic system. Solenoid valves were used to control a micro valve in a pneumatic system. The use of a compressor together with a vacuum pump ensured that one outlet could supply both compression and vacuum pressure. The multi-sample array device was fabricated using conventional photolithography and PDMS casting. The device was composed of a multiplexer, sample array, and rinsing. The multiplexer could control four sample solutions injecting into the sample array chamber. Sample solution not arrayed was removed by DI-water from the rinsing inlet. To prevent trapping of microbubbles in the channel during injection of sample solution into the device, surfactant was added in PDMS solution to serve as a hydrophilic surface treatment. As a result, the device could be used as a sample array for 64 cases, using four samples and three columns of three chambers.