It has been reported that mechanical stimulation takes a role in improving cell growth in skeletal system. Various research groups have been showed their own bioreactors which stimulate ceel-seed three-dimensional scaffold. In this study, we hypothesized that the various conditions of mechanical stimulation would affect cell growth and proliferation. To prove our hypothesis, we designed a custom-made bioreactor capable of applying controlled compression to cell-encapsulated scaffolds. This device consisted of a circulation system and a compression system. Each parts of the bioreactor was fabricated using the rapid prototyping technology. By using the rapid prototyping technology, we can modify and improve the bioreactor very rapidly. For dynamic cell culture, cell-encapsulated agarose gel was fabricated in 2% concentration. We performed dynamic cell culture using this agarose gel and developed bioreactor in 3 days.
