Recently, microalgae have been focused on potential biomass for bio-diesel and biorefinery process. The aim of this paper is to review the biorefinery process including biodesel using microalgae as a microreactor. The state-of-the-art of biodiesel and biorefinery research such as extraction and reaction process as well as byproducts utilization is described. In addition, we suggest possibility for develop bioactive substances and their industrial products from byproducts of microalgae massively obtained after bio-diesel extraction